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. 2016 Jan 11;202(3):1085–1103. doi: 10.1534/genetics.115.185678

Figure 1.

Figure 1

GLD-1 accumulation in the proliferative zone. (A) Schematic of the distal germline from the adult hermaphrodite. The distal proliferative zone, capped by the large somatic distal tip cell (DTC), is ∼20 germ cell diameters (gcd) in length and contains ∼230 germ cells. The proliferative zone is composed of three partially overlapping pools of cells, as indicated. At the proximal end of the proliferative zone, germ cells begin overt meiotic entry. Staining for markers define proliferative zone cells (e.g., nucleoplasmic REC-8 staining) and meiotic prophase cells (e.g., chromosomal HIM-3 staining). First gcd with HIM-3 positive nucleus is start of meiotic entry region (sME) and last REC-8 positive nucleus is end of meiotic entry region (eME). (B) DAPI and REC-8/HIM-3 immunofluorescence image for wild type. (C) Representative DAPI and GLD-1 immunofluorescence image for wild type. Quantified 35-gcd region is depicted as white shaded rectangle. Orange triangles, 1st gcd; red triangles, sME; blue triangles, eME; and white bars are 25 µm. (D) Plot of wild-type GLD-1 levels. DE, distal end. Error bars ± SD. n = 90. Orange dot, base; red dot, sME; blue dot, peak.