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. 2016 Jan 11;202(3):1085–1103. doi: 10.1534/genetics.115.185678

Figure 8.

Figure 8

GLD-1 levels in germ cells irreversibly committed to meiotic entry. (A) Growth scheme of glp-1(bn18) to generate proliferative zone cells identifiable as irreversibly committed to meiosis. (B) DAPI and REC-8/HIM-3 immunofluorescence image of glp-1(bn18) after 4 hr at 25°. (C) DAPI and REC-8/HIM-3 immunofluorescence image of glp-1(bn18) 24 hr at 15° after 4 hr at 25°. A total of 41/42 gonads lacked REC-8 positive nuclei as all proliferative zone cells have entered meiosis. (D) Representative GLD-1 immunofluorescence image of glp-1(bn18) after 4 hr at 25°. Orange triangle, 1st gcd; red triangle, sME; blue triangle, eME; white bars in B–D are 25 µm. (E). Plot of GLD-1 levels. Cyan dashed line extends from base GLD-1 level in glp-1(bn18) after 4 hr at 25°, ∼40%, to the same level in wild type, which occurred at ∼12–13 gcd from distal end. We interpreted this as the approximate position of germ cell irreversible commitment (IC) to meiotic development. (F) Scatterplot of GLD-1 base. **P < 0.01, Sidak’s corrected-multiple comparison. Staining results for analysis of glp-1(bn18) shifted for 3 hr at 25° are shown in Figure S9.