Table 1. Summary of proliferative zone size and cell diameter distance to meiotic entry region.

Genotype	Proliferative zone GCsa mean ± SD (range)	sMEb mean ± SD (range)	eMEc mean ± SD (range)	n
Wild type	246 ± 27 (201–314)	19 ± 2 (16–23)	26 ± 2 (22–31)	30
gld-1 repression
fbf-1(ok91)	226 ± 20 (194–266)	15 ± 2 (12–17)*	22 ± 2 (19–24)*	15
glp-1(bn18)	144 ± 23 (117–191)*	10 ± 2 (7–13)*	15 ± 3 (12–20)*	15
fbf-1(ok224); glp-1(bn18)	134 ± 15 (116–159)*	9 ± 2 (6–12)*	15 ± 3 (9–20)*	15
fbf-2(q738)	296 ± 21 (238–324)*	23 ± 3 (19–28)*	31 ± 3 (26–37)*	15
gld-1 activation
gld-2(q497)	339 ± 37 (265–393)*	21 ± 2 (17–26)*	34 ± 4 (25–42)*	15
gld-3(q730)	283 ± 26 (243–320)*	22 ± 3 (15–29)*	28 ± 4 (24–40)*	15
gld-3(q741)	333 ± 38 (275–430)*	20 ± 3 (15–23)*	28 ± 3 (20–35)*	15
nos-3(oz231)	176 ± 18 (143–203)*	15 ± 2 (11–20)*	23 ± 3 (19–28)*	15
Physiological effect
eat-2(ad465)	110 ± 15 (85–139)*	12 ± 3 (7–17)*	16 ± 2 (12–23)*	15
eat-5(ad464)	160 ± 35 (106–211)*	16 ± 2 (11–21)*	22 ± 3 (17–28)*	15
daf-1(m40)d	154 ± 20 (120–185)*	16 ± 2 (12–20)*	22 ± 2 (19–25)*	15
Putative regulators
lst-1(ok814)	183 ± 19 (140–205)*	17 ± 2 (13–21)*	22 ± 2 (16–25)*	15
sygl-1(tm5040)	137 ± 15 (107–168)*	12 ± 2 (9–16)*	17 ± 2 (14–21)*	15
mir-35 fam(0)e	186 ± 35 (130–252)*	17 ± 3 (12–20)*	23 ± 3 (18–26)*	15

SD, standard deviation.

^*Significantly different than wild type, P-value ≤0.01, Fisher’s uncorrected LSD.

^aGC, germ cells, proliferative zone cell is considered REC-8 positive, HIM-3 negative nucleus.

^bsME, start of meiotic entry region, number of germ cell diameters from distal end to the first HIM-3 positive germ cell.

^ceME, end of meiotic entry region, number of germ cell diameters from distal end to the last REC-8 positive germ cell.

^dStrain maintained at 15°, shifted to 20° at mid-L4 stage.

^eNull(0) for mir-35 family; actual genotype mir-35 thru 41(nDf50) mir-42-45(nDf49).