Table 3. Summary of values obtained from best-fit sigmoidal model.

Genotype	Steepness (95% C.I.)a	CDmaxR (95% C.I.)b	r2c	Comparison of fitd
Wild type	0.13 (0.10–0.14)	14.1 (13.7–14.4)	0.80	N/A
gld-1 repression
fbf-1(ok91)	0.12 (0.10–0.14)	9.6 (9.2–10.1)	0.80	ND
glp-1(bn18)	0.17 (0.12–0.21)	7.1 (6.6–7.5)	0.78	ND
fbf-1(ok224); glp-1(bn18)	0.14 (0.09–0.02)	7.2 (6.1–8.3)	0.61	ND
fbf-2(q738)	0.09 (0.08–0.09)	17.0 (16.3–18.0)	0.82	<0.0001
gld-1 activation
gld-2(q497)	0.09 (0.08–0.10)	14.9 (14.2–15.6)	0.77	<0.0001
gld-3(q730)	0.10 (0.09–0.11)	11.5 (11.1–12.0)	0.83	0.0028
gld-3(q741)	0.11 (0.10–0.13)	13.0 (12.4–13.6)	0.82	0.0565
nos-3(oz231)	0.14 (0.13–0.16)	13.6 (13.0–14.2)	0.80	0.0016
Physiological effect
eat-2(ad465)	0.24 (0.22–0.26)	9.3 (9.0–9.6)	0.85	<0.0001
eat-5(ad464)	0.15 (0.13–0.17)	13.5 (12.6–14.3)	0.67	0.0017
daf-1(m40)	0.15 (0.14–0.17)	11.5 (11.0–12.0)	0.75	<0.0001
Putative regulators
lst-1(ok814)	0.11 (0.09–0.14)	12.0 (11.2–12.8)	0.78	<0.0001
sygl-1(tm5040)	0.24 (0.22–0.27)	8.9 (8.6–9.2)	0.82	<0.0001
mir-35 fam(0)e	0.14 (0.13–0.16)	12.2 (11.8–12.6)	0.79	0.0002

ND, not determined.

^aSteepness, analogous to Hill slope value, but changed to reflect its descriptive rather than functional significance for this study.

^bCDmaxR, position in gcd where GLD-1 level is halfway between top and bottom values (see Figure 7). Interpreted as position where GLD-1 accumulation change is maximum throughout the proliferative zone.

^cr² correlation coefficient for best-fit sigmoidal model for a given genotype compared to experimentally determined GLD-1 levels.

^dSteepness values for each genotype were compared pairwise to the wild-type model by goodness-of-fit test. P < 0.01 considered different. P-value is not calculated when steepness can be described by the same value.

^eNull(0) for mir-35 family; actual genotype mir-35 thru 41(nDf50) mir-42-45(nDf49).