Figure 6.

VAV-1 is required for survival after noxious heat stress. (A) Animals were exposed to 40° heat stress for 20 min, and survival was assessed over the following 4 days. Like plc-3(tm1340) mutants, which are defective for heat stress–induced quiescence (Hill et al. 2014), vav-1 mutants show a loss of survival after exposure to noxious heat. This survival defect is rescued by expression of wild-type vav-1. (B) None of the tested strains showed differences in survival without heat stress exposure. (C) Survival of wild-type animals and vav-1, egl-4(ad450sd), and egl-4(ad450sd); vav-1 animals after 20 min of exposure to 40° heat stress. egl-4(ad450sd) significantly suppresses the survival defect observed in vav-1 mutants. Data are represented as mean ± SEM, and statistical analyses were conducted using repeated-measures two-way ANOVA followed by Tukey posttests. P-values are detailed in File S1.