Figure 3.

Effects of additional glucose on the growth inhibitions and responses induced by cationic amphiphiles. (A) Logarithmic BY4741 cells were layered onto the YPD plates (pH 7.5) containing 2 or 8% glucose. Filter papers containing 3 µl of the drugs (20 mM CPZ, 20 mM TFZ, 200 mM SDS, 72 mM G418, 10 mM TMA12, 500 mM DC, and 500 mM TC) were put onto each plate and incubated for 2 days at 25°. Close-ups of the halos formed by DC and TMA12 are shown in the left panel. (B) The BY4741 strain was grown for 3 days at 25° on 96-well plates containing YPD (2 or 8% glucose, pH 6.0) supplemented with LC and PC (procaine) at the indicated concentrations. (C) Logarithmic cultures (OD₆₀₀= 0.7) of BY4741 grown in YPD (2% Glc, pH 6.0) and in YPD supplemented with additional 4% glucose (6% Glc) and 4% methylglucose (2% Glc + 4% OMG) were treated with 2.5 mM (left) or 10 mM (right) of TC at time zero. The percentages of cells with polarized actin (top) and ALP activities (bottom) are indicated, respectively. TC was omitted from the cell cultures containing each concentration of TC at the indicated times (arrows) and then incubated (without tetracaine) (top panels). (D) Logarithmic cultures of BY4741 cells grown in YPD (2 or 6% glucose, pH 6.0) were treated with TC at 2.5 mM for 4 hr or 10 mM for 15 min, and their polysome profiles were analyzed. The polysome/monosome (P/M) ratios are indicated in each panel.