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. 2016 Mar 11;11(3):e0151224. doi: 10.1371/journal.pone.0151224

Fig 1. Scanned image of an antigen microarray probed with positive control serum.

Fig 1

Array features were spotted in triplicate and then probed with serum with known reactivity to ribosomal phosphoprotein P0. A single Cy3-labelled secondary antibody that detects human IgG/IgM was used. Identity of antigens is indicated by the legend. IgG that is spotted onto the array is detected by the secondary antibody. Multiple reactivities are detected with the positive control serum including ribosomal phosphoprotein P0 (RiboP) (green box), single-stranded DNA (ssDNA) (orange box), double stranded DNA (dsDNA) (purple box) and the heat shock protein GRP78 (blue box). Red boxes indicate placement of six different cellular lysates. Array features are approximately 500 mm in diameter.