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. Author manuscript; available in PMC: 2016 Oct 16.
Published in final edited form as: ACS Chem Biol. 2015 Sep 15;10(10):2405–2414. doi: 10.1021/acschembio.5b00296

Figure 4.

Figure 4

Proline isomer specificity of Fcp1. (A) Kinetic analysis of Fcp1 against native, cis-locked, and trans-locked Pro3 containing phospho-Ser2 peptides. Fcp1 shows comparable and higher activity against native (tomato) and trans-locked (blue) peptides. Activity against cis-locked peptide (goldenrod) is observed but lower than that observed for native and trans-locked substrates. Native and trans-locked data are from three experimental replicates; error bars indicate standard deviation (n = 3). cis-Locked data is from one experimental replicate (n = 1). (B) Fcp1/Pin1 coupled assay. All trials show comparable activity with or without Pin1 supplementation. Error bars indicate standard deviation (n = 3).