Fig. 1.

Effect of DHFR knockdown on EA.hy926 cell proliferation. DHFR knockdown in EA.hy926 cells was accomplished by transfection with specific siRNA targeting at DHFR (DHFR-siRNA1/2) or by non-targeting negative control (NC-siRNA). (A) The interference effect was detected with DHFR antibody (dilution 1:800) by western blot for protein expression, at 72 h post-transfection. β-actin served as internal loading control (dilution 1:8,000). (B) Cell viability was measured by MTT assay on days 1, 3, and 5 post-transfection. Experiments were performed in triplicate and data were given as mean ± SD. Representative figure from one out of three experiments is shown. (C) Optical microscopy photos of cells on days 3 post-transfection. Bar scale at low-left = 100 µm. **vs NC-siRNA, p<0.01.