Fig 1. Representative overlaid chromatograms acquired from intact and denatured male and female mouse urine samples (A), the mass spectra of DTBP obtained from a male mouse urine sample (B) and a synthetic standard (C).

The intensity distributions of DTBP and SBT in the urine samples are shown in the insets (A). Guanidine hydrochloride (GdmCl) was added to urine to denature urinary proteins.