Fig 5. Semi-quantitative RT-PCR analyses showing the impact of FurA depletion, FurA overexpression and iron deprivation on the transcriptional pattern of novel FurA targets.

(A) Total RNA from the wild-type strain PCC 7120 (WT) and the coaR-P_coaT::furA fusion strain AGcoaRFurA (FurA^-) were isolated from cells grown in Co²⁺/Zn²⁺ deprived medium (BG-11_-Co/Zn). (B) Total RNA from the wild-type strain PCC 7120 (WT) and the furA overexpressing strain AG2770FurA (FurA⁺) were isolated from cells grown in standard BG-11 medium (+Fe²⁺) or iron deprived medium BG-11_-Fe (-Fe²⁺). Housekeeping gene rnpB was used as control. Determinations for each gene were performed in the early exponential phase of PCR. Expression analyses of genes furA and isiA were included as controls of experimental conditions. All determinations were performed three times with independent biological samples, and the relevant portion of a representative gel is shown for each gene.