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. Author manuscript; available in PMC: 2016 Aug 1.
Published in final edited form as: J Invest Dermatol. 2016 Feb;136(2):444–452. doi: 10.1016/j.jid.2015.10.061

Figure 3. Constitutive expression of AREG-CTD does not activate ERK signaling in AREG silenced cells.

Figure 3

Parental and AREG-CTD rescue cells were cultured in basal KSFM medium with or without Tet with or without the ErbB tyrosine kinase inhibitor PD158780 (1 μM) or the MEK inhibitor U0126 (10 μM), with and without 100 ng/ml rhAREG. After cell lysis, protein expression was analyzed by western blotting with antibodies against AREG, phospho-ERK and total ERK.