Fig. 4. Enzymatic digestion of cellulose by C. thermocellum exoproteomes.
(A to C) C. thermocellum exoproteomes were combined with Avicel PH101 (A) and DACS (B and C) in a 1.4-ml slurry of 1% cellulose content. Samples were taken at 4, 12, 48, 96 and 120 hours. Enzymes were loaded equally based on milligram of protein per gram of glucan. Percent glucan (A and B) and xylan (C) conversion was calculated by quantifying the amount of cellobiose plus glucose, or xylose by HPLC, converting to polysaccharide equivalents, and dividing the amount by the initial glucan or xylan contents, respectively.