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. Author manuscript; available in PMC: 2016 Nov 3.
Published in final edited form as: Structure. 2015 Sep 10;23(11):1977–1988. doi: 10.1016/j.str.2015.08.008

Table 2.

PtdIns(4,5)P2-Dependent Binding of ASAP1 and PLC δ1 PH Domains to LUVs

Protein KD (µM)
No PthSer +PthSer
ASAP1 PH ND 7 ± 1
PLC δ1 PH   1.3 ± 0.2 1.7 ± 0.2
PZA ND 11 ± 2
PdZA 0.96 ± 0.15 0.73 ± 0.2

Binding of recombinant proteins to LUVs either lacking the anionic lipid PtdSer or containing 75 µM PtdSer and variable concentrations of PtdIns(4,5)P2 was determined as described in Experimental Procedures. Because the concentration of the PLC δ1 recombinant proteins was greater than the estimated KD, the data were fit to quadratic equations. The values presented are from two experiments. The data for ASAP1 PH and PZA were fit to a hyperbolic function to estimate of the KD for the PtdIns(4,5)P2-protein complexes. The values are the average ± SEM for six experiments. ND, not determined.