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Induction of non-apoptotic cell death by irradiation. (a) Measurement of caspase activity and (b) Flow cytometry analysis of cell death at 48h after 10 Gy irradiation in cytochrome c normal (Cyt c+/+) and deficient (Cyt c−/−) cells. (c) Assessment of PI positivity and (d) LDH release at 48h after 15 Gy irradiation in Cyt c−/− cells. Mean SD, * p < 0.01 vs. Cyt c+/+ IR, #p < 0.05 vs. control.
