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. Author manuscript; available in PMC: 2016 Oct 21.
Published in final edited form as: Oncogene. 2015 Aug 3;35(16):2075–2086. doi: 10.1038/onc.2015.269

Figure 3. Exogenous EGF does not restore expression of FoxM1 and its target genes in response to AREG silencing.

Figure 3

N/TERT-TR-shAREG cells were incubated in the presence or absence of Tet with and without 20 ng/ml EGF for 60 h. Gene expression was analyzed by QRT-PCR of total RNA with TaqMan assays for genes indicated in the figure. Data are expressed as percent of untreated controls, mean +/− SEM. Pairwise comparison of the three treatments compared to control showed nominally significant differences (* = P <0.05, ** = P <0.005 and *** = P <0.0005) for all five genes for Tet (n=4) and for TET/EGF (n=3).