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(a) Representative mass spectra for MetA-LDI (green), MetA-C60-SIMS (blue), and C60-SIMS (red), generated by averaging pixels across adjacent imaging rows in biofilm 2; (b) scores plot; and (c) loadings plot from unsupervised PCA of 780 average row spectra from biofilms 1 (•), 2 (×), and 3 (+), and the media control C (O). The gold cluster ions are marked with an asterisk (*) in the MetA-C60-SIMS spectrum of panel (a).
