Fig. 2.

(a) In situ C₆₀-SIMS MS² of a representative media-specific ion, m/z 594.8, produces the successive loss of 101.95 to generate several high-abundance ions that are also found in the MS¹ spectra; (b) MS¹ images of the m/z 186.92–594.72 ion sequence in biofilm 2; and (c) relative abundance change between C₆₀-SIMS and the other two ionization modalities. The white scale bars in (b) represent 1 cm, and the error in (c) was calculated as the relative standard deviation between biofilms with n = 3.