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(a) Predicted fragmentation patterns for the isomeric AQ pairs, and MS2 before and after metallization of (b) m/z 260.2 in an approximately equimolar mixture of PQS and HQNO standards on a silicon wafer, (c) m/z 260.2 from the biofilm surface, and (d) m/z 288.2 from the biofilm surface. These data show that ionization of N-oxide AQs is preferentially suppressed following metallization.
