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. 2016 Mar 8;110(5):1089–1098. doi: 10.1016/j.bpj.2016.01.006

Figure 6.

Figure 6

Loss of Hpca or KCNQ3 channels increases IsAHP sensitivity to muscarinic activation. Superimposed IsAHP traces before (black) and after (red) application of 10 μM of the partial agonist Oxo to (a) control pyramidal neurons (n = 9), (b) PIPKIγ-deficient neurons (n = 5), (c) Kcnq3-null neurons (n = 7), and (d) Hpca-null neurons (n = 9). (e and f) Summary data show the effects of Oxo application on (e) IsAHP amplitude and (f) charge (sAHP charge (QsAHP); the QsAHP was integrated across 18 s from the peak of the IsAHP) under the different conditions. For comparison, we also include the full agonist Oxo-M (1 μM; n = 10), which blocks the IsAHP and QsAHP. Note that in neurons deficient in PIPKIγ, KCNQ3, or Hpca, Oxo produces a block similar to Oxo-M in control neurons. Data represent mean ± SEM. ∗p < 0.05 using the ANOVA Tukey post hoc test. To see this figure in color, go online.