Figure 3.

MreB depolymerization increases DiI-C12 mobility by reducing the capture probability of DiI-C12 molecules. (A) MSD and (B) CPD analysis of Bodipy FL-C12 (cyan) and DiI-C12 (magenta) diffusion in the absence (solid symbols) and presence (open symbols) of A22. (Solid lines) linear (A) or exponential (B) fits (see text and Tables 1 and S2). (C) Individual, long single-molecule trajectory of DiI-C12. (Left panel) Sequence of raw images; (right panel) 2D-Gaussian-rendered single-molecule positions of the trajectory, color-coded according to their occurrence in time from red to black. (Orange) Pre- and succeeding single-molecule events recorded within the observed bacterium. The DiI-C12 molecule diffuses between two capture sites where it essentially remains immobile for 15 frames. Frame numbers are indicated on the left; time interval between two consecutive frames = 12 ms. Scale bar (bottom right), 1 μm. (D) 3D-coordinate transformation of the trajectory of (C) allows for recovering the 3D trajectory plotted on a 3D bacterial model. (E) CPD analysis of the long single-molecule DiI-C12 trajectories using the actual 3D displacements. CPD calculated from trajectory visualized in (C) highlighted in red.