Figure 1.

Absorption changes and dark recovery of PpSB1-LOV in heavy-water buffer. The dark-state spectrum shows a maximum at ∼450 nm (black solid line). After illumination with blue light, the absorbance at 450 nm decreases, indicative of light-state formation (blue dashed line). The absorbance band is fully recovered in the dark (rainbow-colored spectra). For clarity, only every fourth spectrum (time increment, 120 min) is shown. The inset shows the time trace for the absorption recovery at 485 nm. Experimental data (open squares) were fitted to a single-exponential function (red solid line). To see this figure in color, go online.