Skip to main content
. 2016 Feb 11;6(3):292–301. doi: 10.1016/j.stemcr.2016.01.004

Figure 2.

Figure 2

miR-16-1 and miR-191 Antagonize Activin/Smad2 Signaling in mESCs and Repress Mesendoderm Differentiation

(A) Measurement of miR-16-1 and miR-191 levels by RT-PCR after transient transfection with miR mimics or inhibitors. Error bars indicate SD of three independent experiments.

(B) miR-16-1 and miR-191 target sites in the 3′ UTR of Smad2. Red indicates complementarity between miRNA and the target gene. Error bars indicate SD of three independent experiments.

(C) miR-16-1 and miR-191 specifically repress their target in the luciferase assay. Data are shown as mean ± SD of four independent experiments. ∗p < 0.05 ∗∗p < 0.01.

(D) Ectopic expression of miR-16-1/miR-191 inhibits ARE-luc activity. Data are shown as mean ± SD of four independent experiments. ∗p < 0.05 ∗∗p < 0.01.

(E) Total SMAD2 and p-SMAD2 protein levels detected.

(F and G) Relative mRNA levels of genes associated with the three germ layers at EBs D0, D4, and D8 in response to miR-16-1/miR-191 repression (F) or overexpression (G). Error bars indicate SD of three independent experiments.