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. 2016 Feb 25;6(3):284–291. doi: 10.1016/j.stemcr.2016.01.001

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© 2016 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Effect of Osteogenically Induced hBMSC-EVs on Osteogenic Lineage-Related Markers of Homotypic Cells

EVs derived from hBMSCs under osteogenic medium (OM), OM-EVs, or RUNX2 transcriptionally activated in basal medium (BM), RUNX2-EVs, were transferred to uncommitted hBMSCs without further supplementation. BM was set as control. qPCR data for relative gene expression of (A and B) transcription factors, (C) effector, and (D–G) downstream factors, are shown as means ± SEM (three donors, three technical replicates). Letters denote significant differences compared with: a, BM; b, OM; c, RUNX2.