Figure 3.

Transcriptional Perturbations in MODY5-hiPSC-Derived Pancreatic Progenitors

(A) Gene expression of HNF1B, PAX6, PDX1, PTF1A, GATA4, and GATA6 in a non-family control and in iN805 family hiPSCs differentiated for 0, 12, 14, and 17 days. Data are representative of at least two independent hiPSC lines per subject. Replicate experiments are presented. The experiments were replicated at least twice. All error bars indicate SD of three biological replicates in an independent experiment.

(B) Immunostaining for HNF1B and PDX1 on iN805 hiPSCs differentiated for 12 days. Data are representative of at least two independent hiPSC lines per subject. The experiment was replicated at least twice. Scale bar, 200 μm.

(C) The percentage of PDX1⁺ and SOX9⁺ cells after differentiation of iN805 hiPSCs (two to three hiPSC lines per subject were pooled together) for 17 days.

See also Figures S2 and S3.