Figure 5.

Psychosine Promotes Hematopoiesis in the AGM and GPR65 Suppresses Hematopoiesis by Repressing Gata2 Expression

(A) Representative flow cytometric plots of CD31⁺c-KIT⁺ and CD31⁺c-KIT⁺SCA1⁺ cell populations in the AGM after 4 days of culture with 20 μM psychosine.

(B and C) The average percentage of CD31⁺c-KIT⁺ (B) and CD31⁺c-KIT⁺SCA1⁺ (C) cell populations with vehicle or psychosine treatment (6 litters: control [n = 19 embryos]; psychosine [n = 20 embryos]).

(D and E) RT-PCR analysis of Gpr65, Gata2, and Runx1 mRNA (D) and Gata2 primary transcript (E) in FACS-sorted CD31⁺c-KIT⁻ cells (n = 3 independent experiments).

Error bars represent SEM. ^∗p < 0.05; ^∗∗p < 0.01; ^∗∗∗p < 0.001 (two-tailed unpaired Student's t test).