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. 2016 Feb 18;6(3):302–311. doi: 10.1016/j.stemcr.2016.01.009

Figure 2.

Figure 2

Nanog Colonies Emerge Independent of N-Cadherin Expression

(A) Left: NSC on day 0 stained with N-cadherin or E-cadherin. Right: IF images of Nanog colonies from NSC reprogramming cultures at d7 and d11 co-stained with N-cadherin or E-cadherin. Scale bar, 50 μm; d0 N-cadherin = 100 μm. Insets, magnification of field.

(B) Time course of NSC reprogramming showing relative expression of Nanog and genes involved in MET, relative to d0 NSC = 1. Error bars indicate SD of three technical replicates from one representative experiment. ESC, embryonic stem cell.

(C) Quantification of d7 and d11 NANOG colonies co-stained with E-cadherin and N-cadherin. Counts from two independent experiments are stacked and average percentages presented.