Figure 2.

Cell Lineage Distribution of GFP⁺ and GFP⁻ Cells in E11 BRE GFP AGMⁱⁿ and AGM^ex

(A–D) Representative FACS plots showing percentages of HPSC (CD31⁺cKit⁺), EC (CD31⁺cKit⁻), and OC (CD31⁻) within the GFP⁺ and GFP⁻ fractions (A) in vivo AGM (AGMⁱⁿ) and (C) AGM explants (AGM^ex). The absolute number of each cell lineage per E11 AGMⁱⁿ (B) and AGM^ex (D) in GFP⁺ (dark gray) and GFP⁻ (light gray) fractions are compared. (B, n = 6; D, n = 5). Error bars show ±SEM, with p value by t test.

(E) High-magnification image of an immunostained E11 BRE GFP aortic cluster (CD31, magenta; cKit, red; GFP, green). Arrowhead indicates a GFP⁺ and arrow indicates a GFP⁻ hematopoietic cluster cell in vivo (AGMⁱⁿ).

(F) The number of hematopoietic progenitors found in the GFP⁺ (dark gray) and GFP⁻ (light gray) fractions of E11 AGMⁱⁿ CD31and cKit sorted cells (n = 3). Error bars show ±SEM. No differences as measured by Student’s t test.

(G) Fold increase of E11 AGM HPSC, EC, and OC number in the GFP⁺ and GFP⁻ fractions upon culture (ex/in). Significance determined by Student’s t test.