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. 2016 Mar 11;13:8. doi: 10.1186/s12950-016-0117-4

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© Fallows et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Effect of M. leprae on M1 surface marker expression. CD14⁺ cells were seeded in 24-well plates at the concentration of 5x10⁵/well and polarized (M1), or exposed to M. leprae at MOI 5:1 for 5 h prior to polarization (Lep5/M1). Additional conditions included stimulation of monocytes with M. leprae at MOI 5:1 (Lep5) and cells in culture medium alone without stimulation (UN). Changes in the percentages of CCR7⁺ and CD40⁺ cells were evaluated. A two-tail paired Student’s t-test was used for the analysis. Results are representative of 6 experiments (6 individual donors); Histogram of one representative experiment. Dark gray: unstimulated cells; light gray: M1 cells; black line: Lep5/M1. *P ≤ 0.05; **P ≤ 0.001; ***P ≤ 0.0001