Table 2.

Sequences of primers used in nested multiplex PCR for the detection of Mycobacterium spp.

Genes	Primers	Sequence (5’-3’)	Size (bp)	Reference (Primers)
nMTP40 a	MTB2F	CGTTCGGGATGCACTGCG	342	In this study
	MTB2R	CACCCGGCGAATTCGTCAC
nIS6110 b	MTBC2F	CGATCGCCCCATCGACCTACT	500	In this study
	MTBC2R	GGTCGAGTACGCCTTCTTGT
n32kD α-antigen c	NTM2F	CACCCGCAGTTCATCTA	413	In this study
	NTM2R	CGTTGTAGGCGTCCTGG

n Primers used in nested PCR

^aspecific for Mycobacterium tuberculosis

^bspecific for Mycobacterium tuberculosis complex

^cspecific for non-tuberculous mycobacterium