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. 2015 May 16;9(8):1678–1693. doi: 10.1016/j.molonc.2015.04.015

Figure 5.

Figure 5

Generation of OVCAR‐3/TP GFP/mCherry cells with expression of ab429 and c141 cassettes. Total RNA was isolated from OVCAR‐3 GFP/mCherry empty, OVCAR‐3/TP GFP/mCherry empty, OVCAR‐3/TP GFP/mCherry ab429 and OVCAR‐3/TP GFP/mCherry c141 cells 48 h after cell seeding. The expression of (A) miR‐200a (200a), (B) miR‐200b (200b), (C) miR‐429 (429), (D) miR‐200c (200c), and (E) miR‐141 (141) was determined. Representative data of three independent experiments are shown. Significance was determined between the OVCAR‐3/TP GFP/mCherry empty compared to OVCAR‐3 GFP/mCherry empty, and OVCAR‐3/TP GFP/mCherry c141 and OVCAR‐3/TP GFP/mCherry ab429 compared to the OVCAR‐3/TP GFP/mCherry empty cell line (*, P < 0.05; **, P < 0.01).