Rat SMCs were infected with AdGFP (control) or AdSmad3 followed by treatment with solvent or TGF-β (5 ng/mL) for 24 h.
A. Affymetrix Array data[9] showing fold changes and p values (n=3) of Wnt family gene expression after TGF-β/Smad3 treatment versus AdGFP control.
B. Quantitative RT-PCR confirming up-regulation of Wnts 2b, 4, 5a, 9a, and 11. ╪, ≠: P<0.05, each compared to AdGFP control; *: P<0.05, compared to the other 3 conditions; n=3 independent experiments.