Figure 1. TGF-β/Smad3 stimulate the expression of several Wnt family genes in cultured rat aortic smooth muscle cells (SMCs).
Rat SMCs were infected with AdGFP (control) or AdSmad3 followed by treatment with solvent or TGF-β (5 ng/mL) for 24 h.
A. Affymetrix Array data[9] showing fold changes and p values (n=3) of Wnt family gene expression after TGF-β/Smad3 treatment versus AdGFP control.
B. Quantitative RT-PCR confirming up-regulation of Wnts 2b, 4, 5a, 9a, and 11. ╪, ≠: P<0.05, each compared to AdGFP control; *: P<0.05, compared to the other 3 conditions; n=3 independent experiments.