Rat aortic SMCs were treated with β-catenin stabilizer SKL2001 (40 μM), recombinant Wnt4, Wnt9a, or Wnt11 (A), or with Wnt2b or Wnt5a (B) each at a concentration of 500 ng/ml for 48h. Experiments in A and B were performed separately at different times. The doublet β-catenin bands appeared in Experiment A likely because of the use of a different batch (lot) of the same antibody from Abcam. * p<0.05 compared to control, n=3 independent experiments.