Fig. 1.

Hsp90 inhibitor treatment results in a rapid decrease of GR activation as measured by reduction in GR Ser211 phosphorylation. In all experiments, cells were pretreated with dexamethasone (Dex, 100 nM) an hour prior to ganetespib (Gan, 100 nM). a Treatment of MDA-MB-231 cells with Gan resulted in decreased Dex-induced GR Ser211 phosphorylation (pSer211) and total GR protein levels. This figure is a representative of two independent experiments. b Densitometry of GR pSer211. c Total GR levels from Fig. 3a show a significant decrease over time following the addition of Gan to Dex compared to Dex alone (p < 0.05, mixed effects ANOVA based on this experiment and one replicate)