Fig. 5.

Sensitization of TNBC cells to paclitaxel by an Hsp90 inhibitor is reduced following GR depletion. a Timeline for GR depletion experiments. Western blots of GR-depleted (GR shRNA) and GR-intact (control shRNA) b MDA-MB-231 or c SUM159PT cells treated with doxycycline (Dox) 24 h after plating, followed by vehicle (Veh), ganetespib (Gan, 50 nM), paclitaxel (Pac, 10 nM), or Gan/Pac 24 h later. d IncuCyte analysis was then performed, and the bar graph represents the percentage cell death at 72 h and is the average of two independent experiments. Gan/Pac vs. Pac cell death is significantly increased for control cells (light gray bars, p < 0.0001) but not significantly (NS) increased for GR-depleted cells (dark gray bars, p = 0.6). e IncuCyte analysis at 72 h for SUM159PT cells. Gan/Pac vs. Pac cell death is significantly increased for control cells (light gray bars, p < 0.01) but NS increased for GR-depleted cells (dark gray bars, p = 0.1)