Fig. 2.
Normal prostate epithelial and stromal cell lines secrete MCP-1 and express its receptor. A: MCP-1 in the supernatant of PrEC and PrSC by ELISA. PrEC and PrSC secrete 27.0 ± 40.7 and 2,150 ± 615 pg/ml MCP-1, respectively. B: CCR2 expression in both PrEC and PrSC by Western blot. C: RT-PCR analysis of CCR2 variants from PrEC and PrSC. Both cell lines express CCR2b but not CCR2a (Mph, macrophage).