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. Author manuscript; available in PMC: 2017 Mar 1.
Published in final edited form as: Neurotoxicol Teratol. 2016 Jan 28;54:15–21. doi: 10.1016/j.ntt.2016.01.004

Figure 4. tBHQ suppresses mitochondrial respiration in the bEnd.3 cell line.

Figure 4

After 24-hr treatment with various concentration of tBHQ, OCR was recorded by a Seahorse XFe96 flux analyzer (n=8). (A) OCR recording at baseline and subsequent treatment of 1μM oligomycin, 0.5 μM FCCP, and a 1 μM rotenone and antimycin mixture. Basal respiration (B), ATP production (C), maximum respiration (D) and spare capacity (E) were calculated. All experiments were repeated three times and the results indicate the mean ± SEM. *P < 0.05, ***P < 0.001, ****P < 0.00001 compared to control. (One-way ANOVA, Tukey’s test).