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. Author manuscript; available in PMC: 2016 Mar 13.
Published in final edited form as: Biochemistry. 2015 May 6;54(19):2943–2956. doi: 10.1021/acs.biochem.5b00292

Figure 7.

Figure 7

Seeds of the fibril core peptides induce fibrillation of A546T FAS1-4. The propensity of the two core peptides to seed fibrillation of A546T FAS1-4 was analyzed using ThT fluorescence assay. A–C, Averaged fibrillation curves (n = 3) representing A546T FAS1-4 alone and in combination with 10% or 20% fibril seeds of the core peptides alone or in combination incubated for 96 h. Error bars denote the standard deviations. D-F, Seeds of each core peptide alone or in combination incubated in the absence of A564T FAS1-4 (duplicate experiments). The data shows that seeds of both the F515-R533 and the Y571-R588 peptides are able to seed the fibrillation of A546T FAS1-4 in a dose-dependent manner. When A546T FAS1-4 was incubated with seeds of the mixed peptides an enhanced effect was observed. None of the observed effects could be attributed to a background signal for the peptide seeds since the increase in ThT fluorescence observed for the seeds alone (D–F) displayed a progression distinct from of those of the seeding experiments.