. Author manuscript; available in PMC: 2017 Mar 25.

Published in final edited form as: J Chromatogr A. 2015 Oct 26;1439:112–123. doi: 10.1016/j.chroma.2015.10.057

Table 3.

Dependence of analyte recovery on resuspension solvent. Common solvents were tested for their ability to resolvate the analyte after evaporative drying by speed-vac. The combined effects with two protein precipitation solvents, ethanol and acetonitrile, are shown. Protein precipitation with acetonitrile followed by resuspension with 5% MeOH provided the best recovery in this comparison. FA, formic acid.

Solvent	Resuspended In	% Recovery
EtOH	5% MeOH	9.6
	50/50 DMSO / H₂O	13
	0.1% FA / 5% MeOH	17

ACN	5% MeOH	55
	50/50 DMSO / H₂O	17
	0.1% FA / 5% MeOH	54

% Recovery calculated from duplicate analyses