Secondary structural analysis of FtsXECL1Spn. (A) TALOS+ predictions with probable β-strands and α-helices represented by +1 and − 1, respectively. Probabilities are based on TALOS+ probability values ≥ 0.6, with the exception of the β3 strand, in which only the middle residue (V82) meets this criterion. (B) Heteronuclear 15N–{1H} values for 15N, 13C-labeled FtsXECL1Spn. The error bar on each hNOE value was estimated from the noise in the spectra. Gaps correspond to the N85 (unassigned), Y112 (overlapped), P132 and P144.