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. Author manuscript; available in PMC: 2017 Mar 14.
Published in final edited form as: Angew Chem Int Ed Engl. 2016 Feb 23;55(12):4083–4086. doi: 10.1002/anie.201511750

Figure 2.

Figure 2

Incorporation of ncAAs into human histone H3 (A) western blots of H3K9AcK and H3K56AcK variants with anti-histone H3K9AcK and H3K56AcK antibodies. 2.5 μl of the corresponding PURE reaction, or 1 μg of commercial H3, was loaded into each lane. MS/MS spectrum of the (B) H3K56AcK(YQ(AcK)STELLIR) and (C)H3K56ThioAcK(YQ(ThioAcK)STELLIR) tryptic peptides. (D) HDAC assays performed on histone H3K56AcK and H3K56ThioAcK. Deacetylation and de(thio)-acetylation reactions were quantified by western blots of histone H3 with anti-acetyl histone H3K56 antibody. Non-enzymatic reactions were used as time zero. Data points are the average of three experiments, with error bars representing ±1 SD.