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Incorporation of single or multiple ThioAcK residues into histone H3K14 or H3K14/K56. (A) Western blots of single or multiple ThioAcKs on H3 with anti-histone H3 antibodies. 5 μl of the corresponding PURE reaction was loaded into each lane. Lanes 1 and 12, commercial histone H3 (1 μg) as antibiody control. (B) MS/MS spectra of two tryptic peptides STGG(ThioAcK)APR at the site of K14 and YQ(AcK)STELLIR at K56 of the K14ThioAcK/K56AcK digestion.
