Figure 1. Hypertonic stress extends lifespan in the presence of FUdR.
A. Hypertonic stress can extend lifespan at some NaCl concentrations. Restricted mean lifespans for survival curves shown in panel A. Maximal extension was observed at 250 or 350 mM NaCl in the presence of FUdR. Restricted mean lifespans for each group are shown, with n = 48 to 69 animals per condition.
B. Lifespan extension is independent of osmolyte. Osmotic equivalents of NaCl, KCl, glycerol, or sorbitol extend lifespan equivalently in the presence of FUdR. n = 60 to 106 animals per condition.
All experiments were performed at 25°C with 400 µM FUdR.
Error bars indicate S.E.M., * indicates p<0.0001 in log rank test vs. 50 mM NaCl control.
