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. Author manuscript; available in PMC: 2016 Mar 13.
Published in final edited form as: J Mol Cell Cardiol. 2016 Feb 11;92:158–162. doi: 10.1016/j.yjmcc.2016.02.008

Figure 2. Mesodermal SALL1 expression promotes cardiac fate but its continued expression in CPCs suppresses cardiac differentiation.

Figure 2

(A) DOX-inducible SALL1 construct and strategy for temporal SALL1 overexpression in human PSCs (Sall1-TGhiPSc). (B, C) mCherry (B) or SALL1 (C) expression with/without DOX (after 12 hrs, 1d). This level of SALL1 expression is about six-fold higher than endogenous levels (Fig. S2B), which may be appropriate for the assay. Scale bars: 200μm. (D) Relative expression of cardiac transcription factors and myocardial genes after temporal DOX treatment shown in (A). (E) Flow cytometry analysis showing percentages of cTNT/TNI/KDR positive cells after temporal DOX treatment shown in (A).