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. Author manuscript; available in PMC: 2017 Apr 1.
Published in final edited form as: Fungal Genet Biol. 2015 Dec 14;89:102–113. doi: 10.1016/j.fgb.2015.12.002

Figure 4. Domain architecture and expression analysis of chorismate-binding domain proteins in A. fumigatus.

Figure 4

(A) Domain comparison of A. fumigatus TrpE, PabaA and IcsA. Abbreviations: Ant, Anthranilate; Trp, Tryptophan.

(B) Northern expression analysis of trpE and icsA in A. fumigatus wild-type AF293. Ribosomal RNA was visualized by ethidium bromide staining as loading control. A. fumigatus strain AF293 was grown in liquid GMM containing 20mM glutamine as nitrogen source at 37 °C and 250 rpm for 24 h. Then mycelia were collected, transferred into solid GMM and grown in duplicates for the indicated time at 29 °C.