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. Author manuscript; available in PMC: 2017 Apr 1.
Published in final edited form as: Fungal Genet Biol. 2015 Dec 14;89:102–113. doi: 10.1016/j.fgb.2015.12.002

Figure 5. Physiological analysis of A. fumigatus mutants used in this study and the alignment of anthranilate synthases.

Figure 5

(A) Radial growth of the A. fumigatus wild-type strain (WT), ΔtrpE, ΔicsA, double deletion mutant ΔicsAΔtrpE, icsA over-expression strain OE::icsA, double mutant ΔtrpE OE::icsA, ΔtrpE complemented strain trpEC, and site mutants trpES77L and trpES66R,S77L and on solid GMM and GMM amended with 5mM l-tryptophan (Trp) medium at 37 °C.

(B) Alignment of the L*ES*nS regions of anthranilate synthases from various organisms. The conserved motif is indicated on the bottom line. The sequences shown are: Ec-TrpE, Escherichia coli TrpE (CAA23666); St-TrpE, Salmonella enterica TrpE(WP_001194371); Vc-TrpE, Vibrio cholerae TrpE (WP_001030227); Sc-Trp2, Saccharomyces cerevisiae Trp2 (NP_011014); At-ASA1, Arabidopsis thaliana ASA1 (NP_001190231); At-ASA2, Arabidopsis thaliana ASA2 (NP_180530); An-AN3695, Aspergillus nidulans AN3695 (CBF75591); At-AT03262, Aspergillus terreus AT03262 (XP_001212440); Af-TrpE, Aspergillus fumigatus TrpE (XP_751136). Identical residues among the various proteins are indicated by dark shading, and respective amino acid residue numbers are shown at the C-termini. Arrows mark amino acids of Af-TrpE mutated in this study. (C) Quantification of radial growth on solid media GMM and GMM+5mM l-tryptophan (Trp) at 37 °C, dry weight of mycelia from culture in liquid medium GMM and GMM+5mM l-tryptophan (Trp) at 37 °C, and spore production on solid GMM and GMM+5mM l-tryptophan (Trp) at 37 °C. No phenotype was observed for tryptophan-feedback mutants trpES77L and trpES66R,S77L compared with trpEC.