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. Author manuscript; available in PMC: 2016 Mar 13.
Published in final edited form as: Virology. 2014 Jan 29;452-453:52–58. doi: 10.1016/j.virol.2013.12.039

Fig. 2.

Fig. 2

γ-H2AX levels in cells transfected with plasmids expressing VZV ORF61, ORF62, and ORF63. A. MeWo cells were transfected with 4 ug of plasmid DNAs (pCMV-61, pCMV-62, pCI-63 and pMax-GFP) using Lipofectamine 2000. Two days after transfection, the cells were harvested and lysed, and the clarified lysate was immunoblotted with antibodies to γ-H2AX, H2AX, ORF61 protein, IE62, IE63, and β-actin. The experiment was repeated with similar results. B. MeWo cells were cotransfected with 5 ug of plasmid DNAs (pCMV-61 and pCI63, pCMV-62 and pCI-63 or with pMax-GFP) using Lipofectamine 2000 and processed as described in panel A. The transfection efficiency for melanoma cells was ~20% based on GFP expression. The numbers below the H2AX panels indicate the ratio of intensity of the γ-H2AX and H2AX bands in cells transfected with plasmids expressing VZV proteins divided by the ratio of the intensity of the γ-H2AX and H2AX bands in cells transfected with plasmid expressing GFP. Densitometry was performed using Image J software. The experiment was repeated with similar results.