Fig. 5.

Immunoblot of phosphorylated ATM and total ATM expression in melanoma (MeWo) cells infected with wild-type VZV or VZV deleted for ORF67 (ROka67D). MeWo cells were infected with ROka or ROka67D and 48 hr after infection, the cells were lysed and equivalent amounts of cell lysates were immunoblotted with anti-phosphorylated ATM, anti-ATM, or anti-VZV IE63 antibody. The numbers below the ATM panels indicate the ratio of intensity of the phosphorylated ATM and ATM bands in infected cell lysates divided by the ratio of the intensity of the phosphorylated ATM and ATM bands in mock-infected cells. Densitometry was performed using Image J software. The experiment was repeated with similar results.