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. 2016 Mar 12;13:64. doi: 10.1186/s12974-016-0526-6

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© Bowyer et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Activated microglia in septum associated with vasculature after METH. Septal sections from a control and two METH-treated animals were double labeled using DAB labeling of an antibody to RECA1 and TRITC labeling of an antibody to IBA1. The far left panels show the DAB-labeled RECA1 immunoreactivity to vasculature through visible light and the middle panels show the TRITC-labeled IBA1 immunoreactivity to microglia through fluorescent illumination. The three far right panels are a merger of the first two panels. The purple arrows show regions of particular interest (see the “Results” section for details). All panels are of the same magnification