Fig. 3.

LSD1 inhibition promoted differentiation and inhibited cell migration and self-renewal. a Treatment of MV4-11 cells with compounds 1 and 2 for 12 days caused >90 % cell populations expressing high levels of CD14, a cell surface protein characteristic for macrophages/monocytes. b Treatment of MV4-11 cells with 2 led to significantly more cells expressing high levels of CD11b, a cell surface protein for macrophages/monocytes. c Treatment with 2 significantly reduced numbers of MV4-11 cells that can migrate through a membrane with 8-μm pores (* p < 0.05 with respect to the control). d Treatment of human primary leukemia cells (from an MLL-rearranged leukemia patient) with LSD1 inhibitors 1–3 potently inhibited the colony-forming ability with EC₅₀ values of 0.43, 0.52, and 0.48 μM, respectively